Cell Differentiation Research

To identify differentiation of nEPS for applying any disease, we differentiated into three germ layer cells using nEPS cell line. Ectodermal cells formed neuronal cells, mesodermal cells adipocyte, osteoblast and chondrocyte and endodermal cell led to hepatocyte and pancreatic beta cells.

In the field of bioengineering, active research was conducted on applying stem cells in tissue engineering and differentiation mechanism of pluripotent stem cells like embryonic stem cell and induced pluripotent stem cell, and multi-potent adult stem cells like bone marrow derived stromal cell and adipose tissue derived stem cell.

There have been studies on signaling mechanism and differentiation mechanism that induce differentiation of stem cell into specific cells as the differentiation potency of stem cell into diverse cells was discovered. There is also a tissue engineering attempt to create 3D tissues using stem cell. STC Stem Cell Treatment and Research Institute is studying differentiation of pluripotent stem cell derived from the umbilical cord into cells of different body organs, as well as new substances, protein, culture environments and technologies in which such differentiation can occur more efficiently.

Ectodermal differentiation

When nEPS cell was differentiated into ectodermal cell, it was differentiated into shape of a nerve cell. Differentiation was verified by dyeing the cell with nestin, which is only expressed in nerve cells. We were able to find that Nestin 100㎛ and Hoechst Merge differentiated into a nerve cell. (Below – Neurocyte)

cdr01

Endodermal differentiation

When nEPS cell was differentiated into liver cell as endodermal cell, shape of liver cell was changed. Differentiation was verified by dyeing the cell with α-fetrotein, which is only expressed in liver cells. We were able to confirm that the α-fetrotein Hoechst Merge differentiated into a liver cell. (Below – Hepatocyte)

cdr02

Mesodermal differentiation

When nEPS cell was differentiated into liver cell as endodermal cell, shape of liver cell was changed. Differentiation was verified by dyeing the cell with α-fetrotein, which is only expressed in liver cells. We were able to confirm that the α-fetrotein Hoechst Merge differentiated into a liver cell. (Below – Hepatocyte)

cdr03

STC nEPS cell was differentiated into cartilage cell and osteoblast as mesodermal cells. Cartilage cell consists of collagen and polysaccharide polymer substance.

Differentiation into cartilage cell was verified by dyeing the cell with Alcian blue, which represents blue color through combination with polysaccharide having carboxyl sulfate unit at pH of 2.5. Since osteoblast consists of calcium and phosphorus, its differentiation was verified by dyeing the cell with Von kossa, an agent used to dye calcium.